Journal: Graefe's Archive for Clinical and Experimental Ophthalmology

Article Title: A rabbit model for outer retinal atrophy caused by surgical RPE removal

doi: 10.1007/s00417-023-06014-3

Figure Lengend Snippet: List of markers used for immunohistochemistry

Article Snippet: Pan cytokeratin , 1:100 , Bio-Techne, Wiesbaden, Germany , NBP2-44,368–0.1 mg , Monoclonal antibody, Clone PAN-CK , Epithelial cells, RPE.

Techniques: Concentration Assay, Plasmid Preparation

Journal: Graefe's Archive for Clinical and Experimental Ophthalmology

Article Title: A rabbit model for outer retinal atrophy caused by surgical RPE removal

doi: 10.1007/s00417-023-06014-3

Figure Lengend Snippet: Visualization of the treated area, the transition zone, and untreated area as direct comparison by SD-OCT and histology at post-operative day 4. Hematoxylin and eosin (HE) staining already reveals atrophy of the photoreceptor outer segments and the outer nuclear layer (ONL) limited to the scraping site. The inner nuclear layer (INL) is not affected. Immunohistochemistry staining of the rabbit retina shows proliferating cells detected via Ki67 staining (red) which colocalize in part with subretinal microglia/macrophages shown by isolectin B4 staining (yellow). A strong deposition of collagen IV (red) is found around the scraping site beneath the ONL. Pan cytokeratin staining (red) for RPE already appears at the scraping site. Cell nuclei are stained with DAPI (cyan). Scale bar for SD-OCT = 200 µm, for histological images = 100 µm

Article Snippet: Pan cytokeratin , 1:100 , Bio-Techne, Wiesbaden, Germany , NBP2-44,368–0.1 mg , Monoclonal antibody, Clone PAN-CK , Epithelial cells, RPE.

Techniques: Staining, Immunohistochemistry

Journal: OncoTargets and therapy

Article Title: Circulating Tumor Cells as a Screening and Diagnostic Marker for Early-Stage Non-Small Cell Lung Cancer

doi: 10.2147/OTT.S241956

Figure Lengend Snippet: Collection and determination of CTCs. ( A ) CellCollector in vivo CTCs detection system. A medical stainless steel wire with a 2-cm-long functional domain coated with EpCAM antibody and a hydrogel stratum. The sampling probe was inserted into cubital vein peripheral blood through a 20G catheter with the functional domain exposed to the peripheral blood and placed in vivo for 30 min to capture tumor cells. ( B ) Determination of tumor cells by CK7/19/panCK (green channel). Hoechst was used for nuclear counterstaining (blue channel), and white cells were determined by CD45 staining (red channel). PD-L1 expression in CTCs was determined by PD-L1 staining (orange channel). Scale bar: 10 μm. WBC indicates white blood cell. ( C ) Collection and determination of CTCs in the recruited patients by CellCollector. Two CTCs were collected in patient number 36, 3 CTCs in patient number 39, and 1 CTC in patient numbers 42 and 44. P indicates patient. ( D ) Imaging and pathological results for patient number 39. A pulmonary nodule was found in the right lung. The patient was diagnosed with invasive adenocarcinoma by pathology.

Article Snippet: The following antibodies were used for staining: CD45 (EXBIO, clone MEM-28-Alexa Fluor 647), cytokeratin CK7/19/panCK antibody (EXBIO Praha, clone A53-B/A2-Alexa Fluor 488), and PD-L1 (clone PD-L1, Abcam).

Techniques: In Vivo, Functional Assay, Sampling, Staining, Expressing, Imaging